Modulated CMOS camera for fluorescence lifetime microscopy

Modulated CMOS camera for fluorescence lifetime microscopy.

Widefield frequency-domain fluorescence lifetime imaging microscopy (FD-FLIM) is a fast and accurate method to measure the fluorescence lifetime of entire images. However, the complexity and high costs involved in construction of such a system limit the extensive use of this technique. PCO AG recently released the first luminescence lifetime imaging camera based on a high frequency modulated CM...

Pco.flim - Fluorescence Lifetime Imaging Cmos Camera

Modulated electron-multiplied fluorescence lifetime imaging microscope: all-solid-state camera for fluorescence lifetime imaging.

We have built an all-solid-state camera that is directly modulated at the pixel level for frequency-domain fluorescence lifetime imaging microscopy (FLIM) measurements. This novel camera eliminates the need for an image intensifier through the use of an application-specific charge coupled device design in a frequency-domain FLIM system. The first stage of evaluation for the camera has been carr...

Probing subtle fluorescence dynamics in cellular proteins by streak camera based Fluorescence Lifetime Imaging Microscopy

We report the cell biological applications of a recently developed multiphoton fluorescence lifetime imaging microscopy system using a streak camera (StreakFLIM). The system was calibrated with standard fluorophore specimens and was shown to have high accuracy and reproducibility. We demonstrate the applicability of this instrument in living cells for measuring the effects of protein targeting ...

Fluorescence lifetime determination for application in microscopy

A recent development in fluorescence microscopy is fluorescence lifetime imaging microscopy. In this type of microscopy the lifetime of the excited state of the fluorescent molecules is of interest rather than the amount of light emitted. By evaluating the response -emission lightof the system -fluorescent moleculesto a specific input signal -excitation light-, the fluorescent lifetime can be d...